WebJun 1, 2024 · Given the importance of 4-(methylsulfonyl)phenylserine for the synthesis of florfenicol and thiamphenicol, mutations of a l-threonine aldolase from Pseudomonas sp. (l-PsTA) were performed in this study by error-prone PCR and combinatorial mutation. Some beneficial mutants were obtained by screening the mutant library using a stepwise visual ... WebAn inducible phenylserine aldolase (L-threo-3-phenylserine benzaldehyde-lyase, EC 4.1.2.26), which catalyzes the cleavage of L-3-phenylserine to yield benzaldehyde and glycine, was purified...
Preparation of (2R,3S)-β-hydroxy-α-amino acids by use of a …
WebDec 5, 2011 · In addition to this THF-dependent SHMT activity, the enzyme exhibits THF-independent aldolase activity toward β-hydroxyamino acids, producing glycine and aldehydes . This reaction has also attracted attention for its characteristic substrate preference. ... The E a for l-erythro-β-phenylserine is larger by ∼ 10 kJ·mol −1 than that ... Webd-Threonine aldolase is an enzyme belonging to the glycine-dependent aldolases, and it catalyzes the reversible aldol reaction of glycine and acetaldehyde to give d-threonine and/or d-allo-threonine. ... In addition, DL-threo-phenylserine and DL-threo-4-(methylsulfonyl)phenylserine were efficiently resolved with an excellent enantiomeric … topf russisch
Phenylserine aldolase - Wikipedia
WebPhenylserine Next to phenylserine itself, 3,4-dihydroxyphenylserine has been the most popular application of threonine aldolases in preparative synthesis since this compound has been used to treat Parkinson's disease (Table 3.1, entries 151–158). From: Advances in Applied Microbiology, 2014 View all Topics Add to Mendeley About this page WebJan 24, 2015 · Only when an intact, active threonine aldolase is introduced into the deletion strain, the substrate dl - threo -β-phenylserine can be cleaved in benzaldehyde and glycine, and the selection strain grows through the metabolism of benzaldehyde via the β-ketoadipate pathway. The enzyme phenylserine aldolase (EC 4.1.2.26) catalyzes the chemical reaction L-threo-3-phenylserine glycine + benzaldehyde This enzyme belongs to the family of lyases, specifically the aldehyde-lyases, which cleave carbon-carbon bonds. The systematic name of this enzyme class is L-threo-3-phenylserine benzaldehyde-lyase (glycine-forming). This enzyme is also called L-threo-3-phenylserine benzaldehyde-lyase. It … picture of my body for kids